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لينك مطلب | نوشته شده در 84/03/09 ساعت 9:22 توسط .... سهراب محمدی .... |


 

 

 

Lilian C. S. MedeirosI; Patrícia G. L. SperidiãoII; Vera L. SdepanianIII; Ulysses Fagundes-NetoIV; Mauro B. MoraisV

ISpecialist in Nutrition, School of Medicine, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil
IIPh.D.; Visiting professor, School of Medicine, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil
III. Ph.D.; Associate professor, School of Medicine, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil
IVFull professor, School of Medicine, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil
V Professor, School of Medicine, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil

 

 ABSTRACT

OBJECTIVE: To assess the food intake and the nutritional status of children on a cow's milk and cow's milk by-products free diet.
METHODS: Twenty-six children receiving a cow's milk and cow's milk by-products free diet were assessed during their first visit to the Pediatric Gastroenterology Clinic (mean age = 19.1 months). Thirty children with no food restriction (mean age = 16.8 months) were also assessed. The usual daily food intake method was used to make the dietary assessment. The food intake was compared between the groups and in relation to the Dietary Reference Intakes (DRIs). The z-scores for weight/age, height/age and weight/height were used to evaluate the nutritional status.
RESULTS: The cow's milk free diet group presented lower energy (p = 0.005), protein (p < 0.001), lipid (p < 0.001), calcium (p < 0.001) and phosphorous (p < 0.001) intake when compared to the control group. The number of children who had energy, calcium and phosphorous intake below the DRIs was higher in the cow's milk free diet group than in the control group. The z-score means for the cow's milk free diet and control groups were, respectively: height/age -0.81±1.06 vs +0.42±1.25 (p < 0.001), weight/age -1.03±1.21 vs +0.02+0.91 (p < 0.001), and weight/height -0.63+1.08 vs +0.30+1.11 (p = 0.004).
CONCLUSIONS: During the cow's milk and cow's milk by-products exclusion therapy, qualitative and quantitative food intake monitoring must be carried out periodically, so as to prevent inadequacies in meeting nutritional requirements and impairment of growth and development.

Key words: Milk hypersensitivity, nutritional status, food intake, calcium.


 

 

Introduction

Intolerance and allergies to cow's milk predominantly occur during the first 3 years of life. Treatment is based on excluding cow's milk, an important source of nutrients for children on artificial feeding, from the diet.1

Care should be taken to introduce an adequate substitute diet which meets the child's nutritional needs when employing this therapy.2 Some studies have demonstrated reduced ingestion of some nutrients,1,3 in addition to reduced stature3,4 in children on a cow's-milk-free diet when compared to children on a normal diet.

The elimination of cow's milk and cow's milk by-products from the diet could lead to insufficient calcium ingestion1,3,5,6 — an element fundamental to bone health7 — increasing the risks of bone mineralization problems8 Cases of rickets,9 osteopenia and osteoporosis have been described in association with low calcium ingestion over long periods as a result of the elimination of cow's milk and cow's milk by-products from the diet.10

This being said, even in situations in which the elimination diet is recommended as a therapeutic test, nutrient intake and nutritional status assessment is necessary so that possible inadequacies can be detected and corrected.11

The objective of the current study was to evaluate the consumption of nutrients and the nutritional status of children on a diet free from cow's milk and cow's milk by-products in comparison with a group of children, from the same age group and socio-economic level, on a normal diet.

 

Methods

This is a cross-sectional study in which two groups were compared: one on a diet free from cow's milk and cow's milk by-products and the other on a normal diet, i.e. with no medical or therapeutic recommendation to exclude any given food.

A total of 26 children were evaluated, 11 female, 15 male and with a mean age of 19.1 months. All of them had been prescribed a diet free from cow's milk and cow's milk by-products at their first consultation at the Pediatric Gastroenterology Clinic of the Universidade Federal de São Paulo - Escola Paulista de Medicina, during the period between September 2001 and April 2002.

The control group was made up of 30 children, of whom 13 were female and 17 were male, with a mean age of 16.8 months and on normal diet, seen consecutively at the Childcare Clinic of the Jardim Santo Eduardo basic health center, in the municipality of Embu.

Elimination criteria for both groups were: fulltime attendance at a school or day-care center, maternal difficulty filling out the questionnaire on the child's nutrition, consumption of human milk and refusal to participate in the study.

Weight and stature were measured according to recommendations made by Jellife.12 The anthropometric indices employed were z scores for weight/age (W/A), stature/age (S/A) and weight/stature (W/S). In line with World Health Organization recommendations nutritional deficiency was defined as a z score below —2.0 standard deviations.13 The z scores were calculated with the aid of Epi Info version 6.0 which employs the NCHS - National Center for Health Statistics data on weight and stature as its reference norms.14,15

The usual daily diet method was employed with the objective of defining the usual dietary consumption of the child.16,17 During the interview questions were asked about meal times, foods and preparations consumed in addition to the six of the portions consumed during a typical dietary day. In order to define portion sizes photographic records18 and models of utensils (cups, cutlery, plates, babies' bottles) were used. Calculations were performed with the aid of the Sistema de Apoio a Decisão em Nutrição (Nutritional Decision Support System) version 2.5 computer program.19 Nutrient intake was compared between the two groups (children on a diet free from cow's milk and cow's milk by-products and the controls) and also in relation to the National Research Council recommendations, as described in the Dietary References Intakes (DRIs) document.20-23

A standardized questionnaire was administered to both the group on a cow's milk and cow's milk by-products free diet and the control group in order to obtain the following data:

— family income: number of national minimum salaries/month;

— vitamin supplement usage: brand and daily supplement dose;

— duration of exclusive maternal breastfeeding defined as when the child receives only breastmilk and no other liquid or solid with the exception of vitamins, minerals and/or medicines in the form of drops or syrups, in accordance with the World Health Organization.24

For the group on a diet free from cow's milk and cow's milk by-products, in addition to the information already described, the following were also recorded: duration of elimination diet, any other foods excluded and the clinical manifestations that prompted the cow's milk and cow's milk by-products free diet to be prescribed.

Parametric and no parametric tests were used to analyze the results depending on the nature of the variables being studied. Fisher's exact test and the chi-square test were used to compare observed proportions for the two independent groups, i.e. children in the experimental group and the control group. The comparison between continuous variables for the two groups, two independent samples, was performed using the Mann-Whitney tests. The Kruskal-Wallis test and Dunn's multiple comparison test were applied to the analysis of four independent samples for the assessment of calcium. Calculations were performed using the program SigmaStat.25 The cut off for rejection of the null hypothesis was set at = 0.05 or 5%.

Sample size was estimated based on the expectation of insufficient calcium consumption among children on cow's milk and cow's milk by-products free diets. In a similar study in England, 60% of children on a diet free from cow's milk and cow's milk by-products were found to have calcium ingestion below 75% of the standard adopted as parameter, while among those children on normal diets this percentage was 17%.6 The sample size was therefore calculated taking the difference between groups as 43%, a error < 0.05 and statistical power of 80%, thus giving a minimum of 24 individuals in each group.25

The experiment was approved by the Committee for Ethics in Research at the Universidade Federal de São Paulo/Hospital São Paulo. Informed consent was obtained in writing from legal guardians.

 

Results

The clinical manifestations that prompted the free from cow's milk and cow's milk by-products, due to clinical suspicion of allergy or intolerance to this food were: failure to thrive (nine), diarrhea (seven), vomiting or regurgitation (six), blood in feces (six), chronic intestinal constipation (five), dermatitis (five), gastroesophageal reflux (three), bronchitis or wheezing (three). Nine of the patients, 34.6%, presented just one of the clinical manifestations listed, while 57.7% (15/26) exhibited two and 7.7% (two) three or more manifestations.

For the group on cow's milk and cow's milk by-products free diets, the medians (25th and 75th percentiles in parentheses) for age at start of diet and duration of diet were, 9.0 months (4.0-14.1) and 7 months (2.0-13.5) respectively. Foods excluded from diets were as follows:

— cow's milk and cow's milk by-products (n = 14);

— cow's milk, cow's milk by-products, and soy (n = 11);

— cow's milk, cow's milk by-products, soy and orange or red colored foods (n = 1).

General characteristics and anthropometric indices for the two groups are shown in Table 1. Observe that, for all indices, the group on diets free from cow's milk and cow's milk by-products presented mean z scores lower than did the control group, with these differences being statistically significant. Taking individual z score values and a < -2.0 standard deviations cut-off point, the group on cow's milk and cow's milk by-products presented a greater number of children with deficiencies compared with the control group for all indices, although the difference was only statistically significant for the weight/age index (Table 2).

 

 

The group on diets free from cow's milk and cow's milk by-products presented reduced ingestion of energy, proteins and lipids compared with the control group, which differences were statistically significant (Table 3).

 

 

Of the 26 children on cow's milk and cow's milk by-products free diets, 10 (38.5%) were given a soy-based formula and two (7.7%) formula based on hydrolyzed proteins, while the remainder did not receive any cow's milk substitute formula. With respect of nutritional supplements, it was observed that in the group on the cow's milk diet, nine (34.6%) children used a calcium supplement, nine (34.6%) iron supplement, seven (26.9%) vitamin A supplement, eight (30.7%) vitamin D and three (11.5%) vitamin C supplement. It was observed that, of the 30 children in the control group, six (20%) used iron supplement, 15 (50%) vitamin A supplement, 15 (50%) vitamin D supplement and six (20%) vitamin C supplement.

Table 4 presents the median ingestion of calcium, phosphorous, iron and vitamins A, C and D for the two groups, broken down into the contribution made by foods (including formulae) and, separately, the contribution made by foods summed to that made by vitamin and mineral supplements.

 

 

The median calcium and phosphorous intake was significantly greater in the control group. Even when the contribution made by calcium supplementation was taken into account, the calcium intake for the group on diets free from cow's milk and cow's milk by-products presented as diminished. Similar iron consumption was observed for the children on cow's milk and cow's milk by-products free diet and the controls, although when the contribution of foods was summed with that of supplements, intake was greater among the children on diets free from cow's milk and cow's milk by-products than among controls, due to the fact that a proportion of the patients were receiving oral iron therapy (Table 4).

The control group exhibited greater vitamin A consumption, both when dietary contribution was taken in isolation and when it was added to the supplementary contribution, although the observed difference was only statistically significant in the first case (dietary contribution). Vitamin C intake was higher in the group of children on cow's milk and cow's milk by-products free diets, differing significantly from that of the control group (Table 4).

The group of children on diets free from cow's milk and cow's milk by-products were split into three subsets in order to verify calcium consumption: children on formula (12), children using calcium supplementation (seven) and children receiving neither formula nor calcium supplement (seven). Their median calcium intakes were (25th and 75th percentiles in parentheses) were 665.90 mg/day (506.08-837), 423.83 mg/day (215.60-657.13) and 158.82 mg/day (79.67-211.25), respectively. The same value for the control group was 1,017.59 mg/day (739.43-1,210.68). Median calcium intake was analyzed across the four groups with the Kruskal-Wallis test, which indicated statistically significant differences. It was found that children who had received calcium supplementation and those who had received neither supplementation or formula exhibited lower calcium intake than did the control group, according to Dunn's multiple comparison test (p < 0.05), while the group that had received formula did not present a statistically significant difference in comparison with the control group.

Energy and nutrient intake for both groups was compared with the Dietary Reference Intakes, and it was observed that a number of children in each group failed to reach 100% of the recommended levels (Table 5). Observe, in Table 6, that the group on cow's milk free diets has the greatest number of children with intakes below recommended levels for energy, calcium, phosphorous and vitamin D, indicting that formula contributes significantly to the nutrient intake of these children.

 

 

 

 

Discussion

The results allow us to state that the nutrition of these children on diets free from cow's milk and cow's milk by-products presents nutrient deficiencies, in particular in terms of energy and calcium, in relation both to international recommendations and to the control group. It is important to emphasize that, as Table 1 shows, there were no differences between the groups in terms of the distribution of family income, sex, age or prior exclusive natural breastfeeding.

Our study is similar to four experiments performed in Europe, which evaluated the dietary intake of children on diets free from cow's milk with an average age of around two years.1,3-5

The nutrient intake of 16 Norwegian children on cow's milk and cow's milk by-products free diets, was compared with that of children receiving soy or hydrolyzed protein based formulae (n = 6), children who weren't receiving formula (n = 10) and controls (children on diets excluding eggs, but not milk or cow's milk by-products; n = 10). When the groups were compared, the formula was observed to make an important contribution to adequate energy and macronutrient intake. Notwithstanding, calcium intake was lower among children on elimination diets than in the control group, irrespective of the use of formula, which fact is possibly the result of the lower volume of formula ingested by children on the elimination diet when compared with the controls.1

Another study evaluated 19 Finnish children on a diet excluding multiple foods, including cow's milk and cow's milk by-products. Just two of the 19 children were receiving formula and the remainder used calcium supplementation. There were no differences in terms of energy, lipid or carbohydrate intake between the children on elimination diets and the controls. The children on elimination diets did, however, exhibit reduced consumption of protein, phosphorous, magnesium, zinc and calcium.3

Another study performed in Finland assessed 18 children on cow's milk and cow's milk by-products free diets (13 receiving formula and 17 calcium supplementation). No important differences were detected between the nutrient intake of this group of children in comparison with controls, but it was ascertained that formula use did not ensure adequate calcium intake, although, with the use of concurrent calcium supplementation, the children on elimination diets' intake became comparable to that of the control group.4

In England, 23 children on diets excluding multiple foods, 12 of whom received soy or hydrolyzed protein formula, did not exhibit important differences in dietary intake when compared to a control group, with the exception of calcium consumption, which was lower for the group on elimination diets.5

In our experiment, in contrast with what was observed in some of the papers cited,3-5 energy intake was considerably lower among the group on elimination diets. The patients also consumed a statistically significant quantity less of proteins and lipids. This suggests that the group had lower food intake in general and not a deficiency of any particular macronutrient.

The use of formula as a substitute for cow's milk during an elimination diet is a feature that merits attention. The formulae usually used are based on hydrolyzed proteins or soy and represent an important contribution to nutrient ingestion, particularly for infants and small children, taking into consideration the difficulties involved with this age group in substituting cow's milk entirely with solid foods.4 Notwithstanding, it is important to assess formula daily intake, observing level of dilution and volume offered, since some studies have found that the volume of formula consumed daily by children on diets free from cow's milk and cow's milk by-products is significantly lower than the milk intake of children on normal diets,1,4 and in our country there is one further aggravating factor which is the high cost of formulae which could lead to incorrect dilution and/or the offer of insufficient quantities.

Another important factor is the use of soy-based drinks or juices as a substitute for cow's milk. Two of the children on diets free from cow's milk and cow's milk by-products in our study consumed soy-based juice with a low calcium content that does not meet the Dietary Reference Intakes recommendation for calcium.20 There are three case histories in the literature describing rickets in Belgian children aged between 15 and 18 months associated with calcium deficiency resulting from the use of a soy-based drink.26 This type of product is not specifically formulated for the pediatric age group and as such should not be recommended as a substitute for cow's milk.

During a diet excluding cow's milk and cow's milk by-products, a large proportion of daily intake can be supplied by means of supplementation, which should be sufficient to meet the adopted recommendation. The calcium intake level recommended by the Dietary Reference Intakes varies according to age group: 210 mg/day (0 to 6 months), 270 mg/day (7 to 12 months) and 500 mg/day (13 to 36 months).20 Our results demonstrated calcium intake below these levels even when supplementation had been instituted (Table 4). The difficulty in correctly prescribing calcium supplementation may be a result of the existence of many different calcium formulations on the market. The choice of calcium supplement will depend upon its cost and elemental calcium content, which will vary according to the type of calcium salt employed (Table 7). In our clinical practice we use a calcium supplement in solution, composed of calcium gluconate and calcium lactobionate, and whose elemental calcium content is 216 mg/10 ml.

 

 

The elimination of cow's milk and cow's milk by-products is a risk factor for low calcium intake since these foods are the main sources of calcium in food. Observations have been made of inadequate bone mineralization in children subjected to these elimination diets for prolonged periods.8,10

The anthropometric assessment of the patients on cow's milk and cow's milk by-products free diets (Tables 1 and 2), when compared with that for the control group, presented lower mean z scores for weight/age, weight/stature and stature/age. At our health service, research has shown that children with constipation associated with intolerance of cow's milk presented lower z scores for weight/age, weight/stature and stature/age than did children with constipation who were shown not to have cow's milk intolerance following either an elimination diet or a challenge test.29,30 The subset with constipation and confirmed intolerance to cow's milk protein, acquire a special interest in the sense that they do not exhibit symptoms such as vomiting, diarrhea or evidence of intestinal malabsorption which could explain the pondero-statural deficiencies. Other studies have demonstrated reductions in indices for stature/age3,4,31,32 and weight/stature31 in children with food allergies.

Studies of the nutritional status of children on elimination diets are scarce, with some observations suggesting nutritional prejudice, without, however, clearly defining the pathophysiologic mechanisms involved. Inadequate nutrient intake may have influenced the nutritional status of the children assessed in our study, but other authors have not found associations between nutritional status and food consumption.3,4,31 Allergic inflammation that is maintained throughout the elimination diet, whether by continued contact with the allergen resulting from noncompliance with the diet or by an allergic reaction to hypoallergenic formula can cause malabsorption and nutrient loss, impacting on growth.31 If the nutrient loss hypothesis is assumed to be true, current nutritional recommendations may be insufficient to guarantee the adequate nutritional status of allergic children,3 in particular those with atopic dermatitis.11,32. Further studies into this subject are necessary in order to provide more information which would allow greater security in the dietary guidance of patients with cow's milk allergies on elimination diets.

Concluding, in this experiment it was found that children on cow's milk and cow's milk by-products free diets presented reduced calcium and phosphorous intake when compared with recommended standards and also when compared with children on normal diets. The same was true of energy intake, which factor may contribute to the nutritional deficit detected. Our findings reinforce the need to monitor the nutrient intake and nutritional status of children put onto diets free from cow's milk and cow's milk by-products in order to avoid nutrient deficiencies during the elimination diet.

 

Acknowledgements

We are grateful to the staff at the Serviço de Saúde do Jardim Santo Eduardo in the municipality of Embú and to Professors from the Programa de Integração Docente Assistencial, for their help. Also to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

 

References

1. Henriksen C, Eggesbo M, Halvorsen R, Botten G. Nutrient intake among two-year-old children on cow's milk-restricted diets. Acta Paediatr. 2000;89:272-8.

2. Arvola T, Holmberg-Marttila D. Benefits and risks of elimination diets. Ann Med. 1999;31:293-8.

3. Paganus A, Juntunen-Backman K, Savilahti E. Follow-up of nutritional status and dietary survey in children with cow's milk allergy. Acta Paediatr. 1992;81:518-21.

4. Tianen JM, Nuutinen OM, Kalavainen MP. Diet and nutritional status in children with cow's milk allergy. Eur J Clin Nutr. 1995;49:605-12.

5. David TJ, Waddington E, Stanton RHJ. Nutritional hazards of elimination diets in children with atopic eczema. Arch Dis Child. 1984;59:323-5.

6. Devlin J, Stanton RHJ, David TJ. Calcium intake and cows' milk free diets. Arch Dis Child. 1989;64:1183-93.

7. National Institutes Health. Consensus Development Panel on Optimal Calcium Intake. JAMA. 1994;272:1942-8.

8. Hidvégi E, Arató A, Cserháti E, Horváth C, Szabó A, Szabó A. Slight decrease in bone mineralization in cow milk-sensitive children. J Pediatr Gastroenterol Nutr. 2003;36:44-9.

9. Davidovits M, Levy Y, Avramovitz T, Eisenstein B. Calcium-deficiency rickets in a four-year-boy with milk allergy. J Pediatr. 1993;122:249-51.

10. Infante D, Tormo R. Risk of inadequate bone mineralization in diseases involving long-term suppression of dairy products. J Pediatr Gastroenterol Nutr. 2000;30:310-3.

11. Mofidi S. Nutritional management of pediatric food hypersensitivity. Pediatrics. 2003;111(6 Pt 3):1645-53.

12. Jellife DB. Evaluación del estado de nutrición de la comunidad. Genebra: OMS; 1968. p. 201.

13. World Health Organization. Physical status: the use and interpretation of anthropometry. Geneva; 1995. Technical Report Series 854.

14. Dean AG, Dean JA, Coulombier D, Brendel KA, Smith DC, Burton AH, et al. Epi-Info, Version 6.0: a word processor database and statistics program for epidemiology on microcomputers. Atlanta (GE): Center of Disease Control and Prevention; 1994.

15. National Center for Health Statistic. Growth curves for children birth - 18 years: United States Department of Health Education and Welfare, Vital and Health Statistic; 1977 Series 11, Nb. 165.

16. Thompson FE, Byers T. Dietary assessment resource manual. J Nutr. 1994;124 Suppl 11:2245-317.

17. Vasconcelos FAG. Avaliação nutricional de coletividades. Florianópolis: Editora da Universidade Federal de Santa Catarina; 1995. p. 117-8.

18. Zabotto CB, Vianna RPT, Gil MF, Cunha DTO, Moreira MA, Domene SAM, et al. Registro fotográfico para inquéritos dietéticos: utensílios e porções. Goiânia: UFG; 1996.

19. Anção MS, Cuppari L, Tudisco LS, Draibe AS, Sigulem D. Sistema de apoio à decisão em nutrição-versão 2.5. Centro de informática em saúde da Universidade Federal de São Paulo - Escola Paulista de Medicina; 1993.

20. National Research Council (NRC). Dietary Reference Intakes for Calcium, Phosphorus, Magnesium, Vitamin D, and Fluoride. Washington (DC): National Academy Press; 1997.

21. National Research Council (NRC). Dietary Reference Intakes for Vitamin C, vitamin E. Selenium, and Carotenoids. Washington (DC): National Academy Press; 2000.

22. National Research Council (NRC). Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc. Washington (DC): National Academy Press; 2001.

23. National Research Council (NRC). Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids. Washington (DC): National Academy Press; 2002.

24. World Health Organization. Indicators for assessing breast-feeding practices. Geneva; WHO;1991.

26. Legius E, Proesmans W, Eggermont E, Vandamme-Lombaerts R, Bouillon R, Smet M. Rickets due to dietary calcium deficiency. Eur J Pediatr. 1989;148:784-5.

27. Grüdtner VS, Weingrill P, Fernandes AL. Aspectos da absorção no metabolismo do cálcio e vitamina D. Rev Bras Reumatol. 1997;37:143-51.

28. Levenson DI, Bockman RS. A review of calcium preparations. Nutr Rev. 1994;52:221-32.

29. Morais MB. Papel da alimentação na etiopatogenia e no tratamento da constipação intestinal na criança [tese]. São Paulo: UNIFESP-EPM; 1999.

30. Tahan S, Motta MEFA, Goshima S, Fagundes Neto U, Morais MB. Características clínicas de crianças com constipação crônica associada à intolerância à proteína do leite de vaca. 11o Congresso Brasileiro de Gastroenterologia Pediátrica; 2003; Salvador. Anais. 2003. p. 19.

31. Isolauri E, Sütas Y, Salo MK, Isosomppi R, Kaila M. Elimination diet in cow's milk allergy: risk for impaired growth in young children. J Pediatr. 1998;132:1004-9.

32. Christie L, Hine RJ, Parker JG, Burks W. Food allergies in children affect nutrient intake and growth. J Am Diet Assoc. 2002;102:1648-51.

 

 

لينك مطلب | نوشته شده در 84/03/09 ساعت 9:13 توسط .... سهراب محمدی .... |


chloride shift

 

Carbon dioxide is produced by the tissues and diffuses into the plasma. In the plasma:

  • a small proportion becomes bound to plasma proteins forming carbamino compounds
  • a small proportion reacts slowly to form carbonic acid which dissociates to form a hydrogen ion and bicarbonate
  • largest proportion enters the red cell where it rapidly forms carbonic acid:
    • this reaction is catalysed by carbonic anhydrase
    • within the red cell, the carbonic acid dissociates and the hydrogen ion combines with the reduced haemoglobin and the bicarbonate diffuses out of the cell
    • the loss of anionic bicarbonate from the cell is compensated (maintaining electrical neutrality) by an influx of chloride ions (anions) - the so-called 'chloride shift'
  • 5-10% of carbon dioxide is transported via direct binding of carbon dioxide to haemoglobin (carbaminohaemoglobin)
    • production of this compound is increased in conditions of hypoxia and reduced when the haemoglobin is oxygenated

 

 

 

 

 

 

 

 

 

 

 

The chloride shift may facilitate oxygen loading and unloading to/from the hemoglobin from the brown bear (Ursus arctos L.).

Brix O, Thomsen B, Nuutinen M, Hakala A, Pudas J, Giardina B.

Zoological Laboratory, University of Bergen, Norway.

 



1. The oxygen binding properties of hemolyzed bear blood were studied in 0.1 M Tris and 0.1 M Hepes buffer with respect to the possible effects of temperature, pH, pCO2, 2,3-DPG, and chloride ions.

 

 2. There was a significant Bohr shift with a Bohr factor (delta log P50/delta pH) of the magnitude of -0.5. The temperature sensitivity expressed by the apparent heat of oxygenation minus the heat of oxygen in solution was about -8.1 kcal/mol at pH 7.4.

 

 3. Chloride ions decreased the oxygen affinity in the concentration range 50-200 mM, and there was a marked increase in the co-operativity of oxygenation up to a chloride concentration of about 200 mM.

4. There were no effects of pCO2 and 2,3-DPG in the presence of 200 mM Cl-, while in the absence of Cl-, 2,3-DPG had the same effect as 200 mM Cl- at 37 degrees C and pH 7.4.

 5. Our results suggests at least two different binding sites for the chloride ion, one high affinity site which may also bind 2,3-DPG in the absence of chloride, and one or more low affinity sites, which only binds chloride.

 6. The results further show, that a chloride shift of about 33 mM may account for as much as a 40% increase in O2 unloading, without taking into account the additional effect of the Bohr shift.

 

 

 

 

 

 

Chloride Shift

 

     Production of bicarbonate occurs in RBCs

     The H+ combines with Hb

     H+ + Hb ® HbH

      HCO3 - diffuses out  & Cl- are attracted in

     In the lungs we get a reversed chloride shift

http://faculty.ncwc.edu/ddaley/B232%20Respir%20notes.htm

 

 

 

Chloride Shift.

 

 As  is formed it diffuses out of the red cell.  Cl- diffuses into the red cell to maintain electroneutrality.  This is the Chloride Shift or Hamburger Shift.

The chloride shift is rapid and is complete before the cells exit the capillary.

The osmotic effect of the extra  and Cl- in venous red cells causes the venous RBC volume to increase slightly.  For this reason venous hematocrit slightly exceeds arterial hematocrit.

Quantitative summary of CO2 transport.

90% of arterial CO2 stores are carried as  with 5% of the stores carried as dissolved CO2, and 5% of the stores as carbamino compounds.

Of the CO2 added in systemic capillaries, 60% is added as  , 30% is added as carbamino compounds and 10% is added as dissolved CO2.

 

 

 

 

Medical Sciences Program, Indiana University, Bloomington, IN, 47405, USA.

The
chloride shift is the movement of Cl(-) from the plasma into erythrocytes as blood moves from the arterial to the venous end of systemic capillaries. The traditional explanation for the chloride shift emphasizes the causative roles of the rise in Pco(2) and the exclusive presence of carbonic anhydrase within the red blood cell. The purpose of this article is, first, to reexamine two aspects of the chloride shift that we feel are traditionally underemphasized. They are the role of hemoglobin in causing the chloride shift and the affect of the chloride shift on the acid-base status of the blood. Second, we wish to reconcile more recent work with the traditional understanding of the chloride shift. The chloride shift has never been modeled from the perspective of the Stewart strong ion approach. Similarly, the traditional understanding has generally treated Cl(-) as a passive participant in the chloride shift whose role was simply to replace the lost negative charge of the outward moving HCO-3. More recent work has suggested that the ingoing Cl(-) is important for both O(2) unloading and acid-base balance of the blood. We conclude this article with a model of the chloride shift that uses the Stewart approach and, though harmonious with the traditional understanding, highlights the importance of hemoglobin and Cl(-) in the chloride shift.

 

 

In animals, blood flowing through the capillaries of the body tissues gains carbon dioxide. This carbon dioxide is transported to the lungs in one of the three forms: as carbon dioxide dissolved in plasma, as part of a compound formed by bonding to hemoglobin, or as part of a bicarbonate ion. Unlike oxygen, which combines with the iron atoms of hemoglobin molecules, carbon dioxide bonds with the amino groups of these molecules. Consequently, oxygen and carbon dioxide do not directly compete for binding sites -- a hemoglobin molecule can transport both gases at the same time. Carbon dioxide combining with hemoglobin forms a loosely bound compound called carbaminohemoglobin. Only about 15% to 25% of the total carbon dioxide is carried this way. The most important carbon dioxide transport mechanism involves the formation of bicarbonate ions (HCO3-). Carbon dioxide reacts with water to form carbonic acid. This reaction occurs slowly in the blood plasma, but much of the carbon dioxide diffuses into the red blood cells. These cells contain an enzyme, carbonic anhydrase, which speeds the reaction between carbon dioxide and water. The resulting carbonic acid dissociates almost immediately, releasing hydrogen ions and bicarbonate ions. As the bicarbonate ions leave the red blood cells and enter the plasma, chloride ions, which also have negative charges, are repelled electrically, and they move from the plasma into the red blood cells. This reversible exchange of chloride and bicarbonate ions between erythrocytes and plasma to effect transport of carbon dioxide and maintain ionic equilibrium during respiration is known as the chloride shift in animal physiology.

 


In plants, along with manganese, chloride is required for the oxygen evolving reaction of photosynthesis. Cl- is a highly mobile anion with two principal functions: it is both a major counterion, maintaining electrical neutrality across membranes, and one of the principal osmotically solutes in the vacuole. Chloride ion also appears to be required for cell division in both leaves and shoots. Since chloride ion (Cl-) is ubiquitous in nature and highly soluble, it is readily taken up and most plants accumulate chloride ion far in excess of their minimal requirements. It is thus rarely, if ever, deficient. Deficiencies can normally be shown only in very carefully controlled solution culture experiments. Plants deprived of chloride tend to exhibit reduced growth, wilting of the leaf tips, and a general chlorosis.

 

 

 

 

 

لينك مطلب | نوشته شده در 84/03/07 ساعت 20:48 توسط .... سهراب محمدی .... |


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